Measurement
M_GSK3B

Part:BBa_K5088502:Design

Designed by: Dascha Khalfine   Group: iGEM24_Marburg   (2024-07-31)


Measurement construct - GSK3B promoter+5'UTR from T. kok-saghyz


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3709
    Illegal SpeI site found at 1600
    Illegal PstI site found at 4136
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1600
    Illegal PstI site found at 4136
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 460
    Illegal BglII site found at 1522
    Illegal BglII site found at 3712
    Illegal XhoI site found at 2956
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3709
    Illegal SpeI site found at 1600
    Illegal PstI site found at 4136
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3709
    Illegal SpeI site found at 1600
    Illegal PstI site found at 4136
    Illegal NgoMIV site found at 3184
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3398


Design Notes

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Source

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References